| 1 | Tess ten Dolle (Neo) | NED | 44.42 | PB | - |
| 2 | Isa Groenewoud (Neo) | NED | 44.50 | PB | +0.080 |
| 3 | Wieke Roemaat (Neo) | NED | 44.78 | PB | +0.360 |
| 4 | Iris Bosch (Senior) | NED | 46.93 | | +2.510 |
| 5 | Eloise van der Sligte (C2) | NED | 48.26 | | +3.840 |
| 6 | Emma Wolters (Neo) | NED | 48.44 | | +4.020 |
| 7 | Aniek Hesselink (Neo) | NED | 48.49 | SB | +4.070 |
| 8 | Elisa Stenvers (B1) | NED | 48.51 | PB | +4.090 |
| 9 | Juliette Bos (Neo) | NED | 49.03 | SB | +4.610 |
| 10 | Jildou van Felius (A2) | NED | 49.12 | PB | +4.700 |
| 11 | Iris Olde Wolsink (Senior) | NED | 49.18 | | +4.760 |
| 12 | Yuna Broeze (C2) | NED | 49.29 | | +4.870 |
| 13 | Esmee Hesselink (D2) | NED | 50.77 | PB | +6.350 |
| 14 | Isabel van der Weide (C2) | NED | 52.55 | PB | +8.130 |
| 15 | Imke Mogezomp (D2) | NED | 58.27 | | +13.850 |
| 16 | Mar Schouten (D1) | NED | 58.56 | | +14.140 |
| 17 | Iris Tekelenburg (E1) | NED | 58.82 | | +14.400 |
| 18 | Meijs Dikken (E2) | NED | 1:01.12 | PB | +16.700 |
| 19 | Benthe Blaak (E2) | NED | 1:01.32 | PB | +16.900 |
| 20 | Suus ter Braak (E1) | NED | 1:04.77 | PB | +20.350 |
| 21 | Sarah Kalsbeek (E2) | NED | 1:05.56 | PB | +21.140 |
| 22 | Babet Lubbers (E2) | NED | 1:05.69 | | +21.270 |
| 23 | Elisa Lelifeld (E2) | NED | 1:07.81 | PB | +23.390 |
| 24 | Manon Scheperkeuter (n/a) | NED | 1:11.42 | PB | +27.000 |
| 25 | Jorne Kramer (E2) | NED | 1:13.18 | | +28.760 |
| 26 | Myrthe Tekelenburg (D2) | NED | 1:13.71 | PB | +29.290 |
| 27 | Frederique Holtkamp (n/a) | NED | 1:15.95 | PB | +31.530 |
| 28 | Adela Shah (E1) | NED | 1:25.71 | PB | +41.290 |
| DNS | Mafelief Nijhuis (n/a) | NED | DNS | | - |