| 1 | Rose Laliberté-Roy (Senior) | CAN | 39.45 | | - |
| 2 | Béatrice Lamarche (Senior) | CAN | 40.02 | | +0.570 |
| 3 | Léa Tessier (Neo) | CAN | 41.56 | | +2.110 |
| 4 | Rose-Anne Grenier (Senior) | CAN | 41.81 | SB | +2.360 |
| Camille Tremblay (A1) | CAN | 41.81 | | +2.360 |
| 6 | Marie-Felix Guérin (B1) | CAN | 42.19 | PB | +2.740 |
| 7 | Cendrine Levesque (Senior) | CAN | 42.61 | | +3.160 |
| 8 | Laurie Cayer (Neo) | CAN | 42.63 | PB | +3.180 |
| 9 | Molly Flynn (C2) | CAN | 42.99 | SB | +3.540 |
| 10 | Marie Ferlatte (Neo) | CAN | 43.12 | SB | +3.670 |
| 11 | Catherine Lamarche (Neo) | CAN | 43.42 | SB | +3.970 |
| 12 | Ariane Fournier (Neo) | CAN | 43.54 | | +4.090 |
| 13 | Isabella Caicedo Gutierrez (B1) | COL | 43.73 | | +4.280 |
| 14 | Émilie Greenfield (Neo) | CAN | 44.46 | | +5.010 |
| 15 | Oriana Sofia Cardenas Loaiza (B1) | CAN | 44.73 | SB | +5.280 |
| 16 | Mila Boivin (B1) | CAN | 44.91 | SB | +5.460 |
| 17 | Léa Boucher (B2) | CAN | 45.80 | PB | +6.350 |
| 18 | Samuelle Tremblay (C2) | CAN | 46.46 | | +7.010 |
| 19 | Genevieve Robinson (C2) | CAN | 46.85 | | +7.400 |
| 20 | Marion Poirier (A1) | CAN | 47.36 | PB | +7.910 |
| 21 | Katarina Culhane (A1) | CAN | 47.48 | | +8.030 |
| 22 | Eugénie Bernier (B1) | CAN | 47.79 | SB | +8.340 |
| 23 | Alyssa Bouchard (C2) | CAN | 48.45 | | +9.000 |
| 24 | Frédérique-Olivia Laporte (C2) | CAN | 48.67 | | +9.220 |
| 25 | Cassandre Desrosiers (C2) | CAN | 48.79 | PB | +9.340 |
| 26 | Xingyan Han (C2) | CAN | 49.53 | | +10.080 |
| 27 | Jade Pelletier (C2) | CAN | 49.63 | PB | +10.180 |
| 28 | Adella Akanko (B1) | CAN | 49.91 | | +10.460 |
| 29 | Sarah-Maude Labrie (n/a) | CAN | 50.17 | SB | +10.720 |
| 30 | Isa-Anne Touchette (n/a) | CAN | 50.48 | SB | +11.030 |
| 31 | Renata Kenzie (B1) | CAN | 50.52 | | +11.070 |
| 32 | Genevive Lemieux (M45) | CAN | 51.04 | PB | +11.590 |
| 33 | Charlotte Roussel (n/a) | CAN | 51.07 | SB | +11.620 |
| 34 | Mirthe De Haan (C2) | NED | 51.11 | | +11.660 |
| 35 | Nora Munro (C2) | CAN | 51.22 | | +11.770 |
| 36 | Guylaine Larouche (M65) | CAN | 52.29 | | +12.840 |
| 37 | Elisabeth Morin (C2) | CAN | 53.95 | PB | +14.500 |
| 38 | Grace Weinstein (C2) | USA | 54.30 | PB | +14.850 |
| 39 | Chloe Boudreau (B2) | CAN | 55.43 | | +15.980 |
| 40 | Charlotte Babin (C2) | CAN | 1:07.11 | | +27.660 |