| 1 | Karin Kalker (Senior) | NED | 43.12 | | - |
| 2 | Marleen de Kroon (B1) | NED | 44.01 | | +0.890 |
| 3 | Michelle Buskermolen (Neo) | NED | 45.47 | SB | +2.350 |
| 4 | Nicole van Tol (C2) | NED | 46.09 | | +2.970 |
| 5 | Eline van der Zwaard (Neo) | NED | 46.15 | SB | +3.030 |
| 6 | Nicky van Leeuwen (C1) | NED | 46.94 | | +3.820 |
| 7 | Lieneke de Kroon (A2) | NED | 47.19 | SB | +4.070 |
| 8 | Karin Bos (A2) | NED | 47.28 | SB | +4.160 |
| 9 | Nathaly van Lammeren (B1) | NED | 47.54 | | +4.420 |
| 10 | Saskia Rewijk (B1) | NED | 48.03 | | +4.910 |
| 11 | Sandra Rodenburg (C1) | NED | 48.38 | SB | +5.260 |
| 12 | Iris Wijfje (C2) | NED | 48.63 | SB | +5.510 |
| 13 | Kim Eveleens (C1) | NED | 48.91 | | +5.790 |
| 14 | Sara Colijn (B1) | NED | 48.97 | PB | +5.850 |
| 15 | Jacqueline Wierenga (Neo) | NED | 49.42 | SB | +6.300 |
| 16 | Claudia Verkerk (C2) | NED | 49.82 | | +6.700 |
| 17 | Moniek Langhout (B2) | NED | 50.04 | | +6.920 |
| 18 | Paula Groen in 't Woud (B2) | NED | 50.40 | SB | +7.280 |
| 19 | Shanna Herweijer (B1) | NED | 53.25 | SB | +10.130 |
| 20 | Jet Maasbach (C1) | NED | 1:01.11 | | +17.990 |
| DNS | Manouk van Tol (A1) | NED | DNS | | - |