| 1 | Sophie Muir (Senior) | AUS | 39.43 | | - |
| 2 | Linda de Vries (Neo) | NED | 40.77 | SB | +1.340 |
| 3 | Brecht Kramer (Neo) | NED | 41.08 | | +1.650 |
| 4 | Claudia Wallin (Neo) | SWE | 41.14 | | +1.710 |
| 5 | Manon Kamminga (A1) | NED | 41.65 | | +2.220 |
| 6 | Brooke Lochland (A2) | AUS | 43.01 | NRJ | +3.580 |
| 7 | Marit Jansen (Senior) | NED | 45.98 | SB | +6.550 |
| 8 | Leonie Lubbinge (Neo) | NED | 46.68 | SB | +7.250 |
| 9 | Isabelle Vos (Neo) | NED | 47.23 | | +7.800 |
| 10 | Jildau van der Weij (Senior) | NED | 48.88 | | +9.450 |
| 11 | Jannie Bootsma-Zoodsma (M40) | NED | 48.96 | | +9.530 |
| 12 | Thea Kroontje (M65) | NED | 49.78 | | +10.350 |
| 13 | Elise Laan (M50) | NED | 50.45 | | +11.020 |
| 14 | Goikje Salverda-Postma (M35) | NED | 51.52 | | +12.090 |
| 15 | Martine Reijenga (Neo) | NED | 51.59 | | +12.160 |
| 16 | Anny Feddema-Berga (M45) | NED | 52.07 | | +12.640 |
| 17 | Anneke Fransman-Balster (M60) | NED | 52.21 | SB | +12.780 |
| 18 | Ruurdtje Oppedijk (A1) | NED | 56.94 | | +17.510 |
| 19 | Wiep Folkerts (M60) | NED | 1:07.57 | | +28.140 |