| 1 | Annette Gerritsen (Neo) | NED | 38.68 | | - |
| 2 | Marianne Timmer (Senior) | NED | 39.01 | | +0.330 |
| 3 | Margot Boer (Neo) | NED | 39.08 | | +0.400 |
| 4 | Paulina Wallin (Senior) | SWE | 39.64 | | +0.960 |
| 5 | Paulien van Deutekom (Senior) | NED | 39.67 | | +0.990 |
| 6 | Claudia Pechstein (Senior) | GER | 39.88 | | +1.200 |
| 7 | Ireen Wüst (Neo) | NED | 39.95 | | +1.270 |
| 8 | Laurine van Riessen (Neo) | NED | 40.14 | | +1.460 |
| 9 | Renate Groenewold (Senior) | NED | 40.21 | SB | +1.530 |
| 10 | Jekaterina Lukojanova (Senior) | RUS | 40.42 | | +1.740 |
| 11 | Hedvig Bjelkevik (Senior) | NOR | 40.60 | | +1.920 |
| 12 | Claudia Wallin (Neo) | SWE | 40.79 | | +2.110 |
| 13 | Aleksandra.88 Mikhajljova (Neo) | RUS | 41.13 | | +2.450 |
| 14 | Kristina Kulesjova (A1) | RUS | 41.53 | | +2.850 |
| 15 | Mari Hemmer (Senior) | NOR | 42.52 | | +3.840 |
| 16 | Anzjelika Gavrilova (Senior) | KAZ | 42.71 | | +4.030 |
| 17 | Heather Richardson (A2) | USA | 43.47 | | +4.790 |
| 18 | Kelly Gunther (Neo) | USA | 43.48 | | +4.800 |
| 19 | Stephanie Combs (A1) | USA | 44.62 | | +5.940 |
| 20 | Erin DiJulio (Senior) | USA | 45.72 | | +7.040 |
| 21 | Sara Nielson (A1) | USA | 48.28 | | +9.600 |
| 22 | Shauna Scott (M35) | USA | 52.89 | SB | +14.210 |