| 1 | Francine Kronenberg (Senior) | NED | 53.12 | SB | - |
| 2 | Nynke Vredenbregt (Neo) | NED | 53.99 | SB | +0.870 |
| 3 | Robin van der Steen (Neo) | NED | 55.40 | SB | +2.280 |
| 4 | Eline Bekkers (Neo) | NED | 55.42 | SB | +2.300 |
| 5 | Mariska van den Hove (Neo) | NED | 1:01.96 | | +8.840 |
| 6 | Katja Heidweiller (Neo) | NED | 1:03.37 | | +10.250 |
| 7 | Lydia Overtoom (Senior) | NED | 1:03.72 | | +10.600 |
| 8 | Janneke Hulsen (Neo) | NED | 1:05.00 | SB | +11.880 |
| 9 | Vera Mourits (Senior) | NED | 1:07.14 | PB | +14.020 |
| 10 | Lotte van der Velde (Neo) | NED | 1:08.59 | SB | +15.470 |
| 11 | Laurien Waaijer (Neo) | NED | 1:13.12 | | +20.000 |
| 12 | Iris Nuijten (Neo) | NED | 1:13.79 | | +20.670 |
| 13 | Heleen Ten Have (Neo) | NED | 1:24.77 | SB | +31.650 |
| DNS | Naomi.96 De Vries (Neo) | NED | DNS | | - |