| 1 | Danique van Rooden (B2) | NED | 44.84 | | - |
| 2 | Milou Butter (Neo) | NED | 45.13 | | +0.290 |
| 3 | Milou van Leeuwen (Neo) | NED | 45.45 | SB | +0.610 |
| 4 | Renske van der Veer (Neo) | NED | 45.67 | | +0.830 |
| 5 | Lara Dingjan (A2) | NED | 45.75 | | +0.910 |
| 6 | Stefanie Koot (B1) | NED | 45.77 | | +0.930 |
| 7 | Anouk Könst (A1) | NED | 45.87 | | +1.030 |
| Emilie Fransen (B1) | NED | 45.87 | | +1.030 |
| 9 | Britt van Wijk (B1) | NED | 47.60 | | +2.760 |
| 10 | Annette Laarhoven (B2) | NED | 48.14 | PB | +3.300 |
| 11 | Kersty Heeremans (A2) | NED | 48.31 | SB | +3.470 |
| 12 | Juul Jansen (B1) | NED | 49.07 | SB | +4.230 |
| 13 | Charline Coates-Kwadrin (B2) | GBR | 49.09 | SB | +4.250 |
| 14 | Sanne Ganzinga (A1) | NED | 49.18 | SB | +4.340 |
| 15 | Merit de Bruin (A1) | NED | 49.21 | | +4.370 |
| Noa Bierens (B1) | NED | 49.21 | SB | +4.370 |
| 17 | Sandra Könst (M50) | NED | 49.76 | | +4.920 |
| 18 | Anouk Rodenburg (B1) | NED | 49.85 | | +5.010 |
| 19 | Evy van Duijn (B1) | NED | 49.93 | | +5.090 |
| 20 | Nadine Bliekendaal (B2) | NED | 50.26 | PB | +5.420 |
| 21 | Emma Hartveld (A2) | NED | 50.27 | | +5.430 |
| 22 | Amy Valentijn (B1) | NED | 50.35 | | +5.510 |
| 23 | Sylvie van den Heuvel (A2) | NED | 50.47 | SB | +5.630 |
| 24 | Valerie Nijman (A1) | NED | 50.74 | SB | +5.900 |
| 25 | Mette van Leeuwen (B2) | NED | 52.44 | SB | +7.600 |
| 26 | Wieteke Huiberts (A2) | NED | 54.81 | SB | +9.970 |
| 27 | Dana de Bruin (B1) | NED | 55.91 | SB | +11.070 |
| 28 | Mirthe Visser (B1) | NED | 56.62 | PB | +11.780 |
| 29 | Carla van Zantvliet (M55) | NED | 57.12 | | +12.280 |
| 30 | Jette Heck (A2) | NED | 57.37 | PB | +12.530 |
| WDR | Iris Meerhoff (Senior) | NED | WDR | | - |