| 1 | Sterre van Schaik (A2) | NED | 41.81 | | - |
| 2 | Tess Postma (A2) | NED | 42.27 | SB | +0.460 |
| 3 | Sofia Schilder (A1) | NED | 42.74 | | +0.930 |
| 4 | Emma Koster (Neo) | NED | 44.67 | | +2.860 |
| 5 | Danique van Rooden (B2) | NED | 44.72 | | +2.910 |
| 6 | Laura Linssen (A2) | NED | 44.78 | | +2.970 |
| 7 | Zhara Möllers (B2) | NED | 45.06 | | +3.250 |
| Fabienne Maarse (B1) | NED | 45.06 | | +3.250 |
| 9 | Mimi van Hall (B2) | NED | 45.35 | | +3.540 |
| 10 | Maartje Heine (Senior) | NED | 45.83 | SB | +4.020 |
| 11 | Roosmarijn Hoekstra (Neo) | NED | 46.59 | SB | +4.780 |
| 12 | Renske van der Veer (Neo) | NED | 46.72 | SB | +4.910 |
| 13 | Tessa Dijksman (Senior) | NED | 47.47 | | +5.660 |
| 14 | Merit de Bruin (A1) | NED | 48.02 | | +6.210 |
| 15 | Britt van Wijk (B1) | NED | 48.12 | SB | +6.310 |
| 16 | Aranka Keur (M40) | NED | 48.23 | SB | +6.420 |
| 17 | Leanne Molenaar (Neo) | NED | 48.63 | | +6.820 |
| 18 | Fleur Kleijne (Senior) | NED | 49.29 | SB | +7.480 |
| 19 | Evy van Duijn (B1) | NED | 49.60 | | +7.790 |
| 20 | Amy Valentijn (B1) | NED | 49.62 | SB | +7.810 |
| 21 | Charline Coates-Kwadrin (B2) | GBR | 49.78 | | +7.970 |
| 22 | Annette Laarhoven (B2) | NED | 50.06 | | +8.250 |
| 23 | Sylvie van den Heuvel (A2) | NED | 50.82 | SB | +9.010 |
| 24 | Noa Bierens (B1) | NED | 50.88 | | +9.070 |
| 25 | Valerie Nijman (A1) | NED | 51.08 | SB | +9.270 |
| 26 | Emma Hartveld (A2) | NED | 51.23 | | +9.420 |
| 27 | Suus van der Slot (A1) | NED | 51.49 | SB | +9.680 |
| 28 | Nadine Bliekendaal (B2) | NED | 52.22 | | +10.410 |
| 29 | Anne Lubbers (B1) | NED | 52.76 | SB | +10.950 |
| 30 | Roxanne Boers (Senior) | NED | 53.76 | SB | +11.950 |
| 31 | Iris Meerhoff (Senior) | NED | 54.01 | | +12.200 |
| 32 | Pien Lodder (Neo) | NED | 54.30 | SB | +12.490 |
| 33 | Wieteke Huiberts (A2) | NED | 56.51 | SB | +14.700 |
| 34 | Dana de Bruin (B1) | NED | 56.83 | | +15.020 |
| 35 | Jette Heck (A2) | NED | 59.14 | SB | +17.330 |
| 36 | Ramona van Leeuwen-van Noort (M50) | NED | 1:01.05 | | +19.240 |