| 1 | Stefanie Koot (B1) | NED | 45.46 | SB | - |
| 2 | Lara Dingjan (A2) | NED | 45.70 | SB | +0.240 |
| 3 | Lotte de Bock (A1) | NED | 45.73 | | +0.270 |
| 4 | Milou van Leeuwen (Neo) | NED | 45.80 | | +0.340 |
| 5 | Milou Butter (Neo) | NED | 45.86 | SB | +0.400 |
| 6 | Shelley Blaauw (A2) | NED | 46.08 | SB | +0.620 |
| 7 | Maureen Hagen (A1) | NED | 46.20 | SB | +0.740 |
| 8 | Kristel van Abswoude (Senior) | NED | 47.45 | SB | +1.990 |
| 9 | Leanne Molenaar (Neo) | NED | 47.72 | SB | +2.260 |
| 10 | Merit de Bruin (A1) | NED | 47.84 | SB | +2.380 |
| 11 | Monique Boerema (M60) | NED | 48.00 | SB | +2.540 |
| 12 | Aranka Keur (M40) | NED | 48.53 | SB | +3.070 |
| 13 | Iris Meerhoff (Senior) | NED | 48.90 | SB | +3.440 |
| 14 | Charline Coates-Kwadrin (B2) | GBR | 49.21 | SB | +3.750 |
| 15 | Jamy Blaauw (B2) | NED | 49.27 | SB | +3.810 |
| 16 | Evy van Duijn (B1) | NED | 49.60 | SB | +4.140 |
| 17 | Kersty Heeremans (A2) | NED | 49.69 | SB | +4.230 |
| 18 | Annette Laarhoven (B2) | NED | 50.03 | SB | +4.570 |
| 19 | Emma Hartveld (A2) | NED | 50.30 | SB | +4.840 |
| 20 | Wende Heeremans (B1) | NED | 50.84 | SB | +5.380 |
| 21 | Amy Valentijn (B1) | NED | 51.64 | SB | +6.180 |
| 22 | Sylvie van den Heuvel (A2) | NED | 51.97 | SB | +6.510 |
| 23 | Melanie van der Zalm (Neo) | NED | 52.80 | SB | +7.340 |
| 24 | Roxanne Boers (Senior) | NED | 53.79 | SB | +8.330 |
| 25 | Maité Stoete (M50) | NED | 54.67 | SB | +9.210 |
| 26 | Pien Lodder (Neo) | NED | 55.10 | SB | +9.640 |
| 27 | Dana de Bruin (B1) | NED | 56.45 | SB | +10.990 |
| 28 | Mirthe Visser (B1) | NED | 57.62 | SB | +12.160 |
| 29 | Ramona van Leeuwen-van Noort (M50) | NED | 1:00.46 | SB | +15.000 |
| 30 | Tjitske Kikstra (Neo) | NED | 1:01.10 | | +15.640 |
| DNS | Nadine Bliekendaal (B2) | NED | DNS | | - |