| 1 | Claretta Caravita (Senior) | NED | 42.25 | SB | - |
| 2 | Rianne Koot (Neo) | NED | 44.82 | SB | +2.570 |
| 3 | Zhara Möllers (B2) | NED | 45.04 | SB | +2.790 |
| 4 | Emma Koster (Neo) | NED | 45.15 | SB | +2.900 |
| 5 | Danique van Rooden (B2) | NED | 45.80 | | +3.550 |
| 6 | Lara Dingjan (A2) | NED | 46.13 | SB | +3.880 |
| 7 | Maartje Heine (Senior) | NED | 46.22 | | +3.970 |
| 8 | Anouk Könst (A1) | NED | 46.28 | SB | +4.030 |
| 9 | Lotte de Bock (A1) | NED | 46.42 | | +4.170 |
| 10 | Milou Butter (Neo) | NED | 46.43 | SB | +4.180 |
| 11 | Renske van der Veer (Neo) | NED | 47.54 | SB | +5.290 |
| 12 | Tessa Dijksman (Senior) | NED | 47.60 | SB | +5.350 |
| 13 | Britt van Wijk (B1) | NED | 48.18 | SB | +5.930 |
| 14 | Leanne Molenaar (Neo) | NED | 48.82 | | +6.570 |
| 15 | Merit de Bruin (A1) | NED | 48.84 | SB | +6.590 |
| 16 | Aranka Keur (M40) | NED | 49.36 | | +7.110 |
| 17 | Charline Coates-Kwadrin (B2) | GBR | 50.13 | SB | +7.880 |
| 18 | Iris Meerhoff (Senior) | NED | 50.26 | SB | +8.010 |
| 19 | Anouk Rodenburg (B1) | NED | 50.29 | SB | +8.040 |
| 20 | Kersty Heeremans (A2) | NED | 50.34 | | +8.090 |
| 21 | Fleur Kleijne (Senior) | NED | 50.54 | SB | +8.290 |
| 22 | Evy van Duijn (B1) | NED | 50.56 | | +8.310 |
| 23 | Annette Laarhoven (B2) | NED | 51.08 | SB | +8.830 |
| 24 | Noa Bierens (B1) | NED | 51.11 | SB | +8.860 |
| 25 | Amy Valentijn (B1) | NED | 52.69 | | +10.440 |
| 26 | Emma Hartveld (A2) | NED | 52.70 | | +10.450 |
| 27 | Valerie Nijman (A1) | NED | 52.88 | SB | +10.630 |
| 28 | Nadine Bliekendaal (B2) | NED | 53.05 | | +10.800 |
| 29 | Mette van Leeuwen (B2) | NED | 54.05 | | +11.800 |
| 30 | Sylvie van den Heuvel (A2) | NED | 54.42 | | +12.170 |
| 31 | Maité Stoete (M50) | NED | 55.38 | SB | +13.130 |
| 32 | Pien Lodder (Neo) | NED | 56.00 | | +13.750 |
| 33 | Dana de Bruin (B1) | NED | 57.70 | | +15.450 |
| 34 | Tjitske Kikstra (Neo) | NED | 1:00.05 | | +17.800 |
| 35 | Ramona van Leeuwen-van Noort (M50) | NED | 1:03.28 | SB | +21.030 |
| 36 | Anne Lubbers (B1) | NED | 1:04.96 | | +22.710 |
| WDR | Mirthe Visser (B1) | NED | WDR | | - |