| 1 | Leila Meijer (B2) | NED | 41.99 | | - |
| 2 | Kim Zuiver (A2) | NED | 43.23 | SB | +1.240 |
| 3 | Madelief de Jong (A2) | NED | 43.56 | | +1.570 |
| 4 | Michelle van den Hoek (B2) | NED | 43.95 | | +1.960 |
| 5 | Anouk Timan (Neo) | NED | 44.73 | | +2.740 |
| 6 | Maartje Pont (Neo) | NED | 44.74 | SB | +2.750 |
| 7 | Tessa Timmerman (A2) | NED | 44.88 | | +2.890 |
| 8 | Famke Gerritsen (A1) | NED | 45.10 | SB | +3.110 |
| 9 | Famke Wilmink (Neo) | NED | 46.26 | SB | +4.270 |
| 10 | Maartje Heine (Neo) | NED | 46.40 | | +4.410 |
| 11 | Inger van Dok (A1) | NED | 46.42 | | +4.430 |
| 12 | Kristel van Abswoude (A2) | NED | 46.79 | | +4.800 |
| 13 | Masja Keesman (C1) | NED | 46.94 | | +4.950 |
| 14 | Iris Daman (B1) | NED | 47.33 | | +5.340 |
| 15 | Milou van Leeuwen (B1) | NED | 47.54 | | +5.550 |
| 16 | Renske van der Veer (B1) | NED | 47.69 | | +5.700 |
| 17 | Zus de Kruijk (C2) | NED | 47.75 | | +5.760 |
| 18 | Eva Peters (Senior) | NED | 47.77 | | +5.780 |
| 19 | Leanne Molenaar (B1) | NED | 47.83 | SB | +5.840 |
| 20 | Henriëtte Goede (M50) | NED | 47.98 | | +5.990 |
| 21 | Milou Rijksbaron (B1) | NED | 48.05 | | +6.060 |
| 22 | Esther Pletting (Senior) | NED | 48.20 | SB | +6.210 |
| 23 | Fenna de Graaf (D2) | NED | 48.38 | SB | +6.390 |
| 24 | Marit Dee (B2) | NED | 48.56 | SB | +6.570 |
| 25 | Ciska de Ree (M30) | NED | 48.82 | | +6.830 |
| 26 | Sanne Roos (C2) | NED | 48.83 | | +6.840 |
| 27 | Demi Könst (C2) | NED | 48.86 | SB | +6.870 |
| 28 | Lara Dingjan (C1) | NED | 49.16 | | +7.170 |
| 29 | Anouk Maerten-van der Kieft (Senior) | NED | 49.29 | SB | +7.300 |
| 30 | Anja van Diest-van der Poel (M50) | NED | 50.76 | SB | +8.770 |
| 31 | Zhara Möllers (D1) | NED | 50.77 | | +8.780 |
| 32 | Sanne Ganzinga (D2) | NED | 50.94 | SB | +8.950 |
| 33 | Daisy Kolk (Senior) | NED | 50.98 | | +8.990 |
| 34 | Romy Busch (C1) | NED | 52.03 | | +10.040 |
| 35 | Roos Aarsen (C1) | NED | 52.07 | | +10.080 |
| 36 | Eeke Emilie Weenink (C1) | NED | 52.47 | | +10.480 |
| 37 | Elise van der Sluis (B2) | NED | 53.21 | | +11.220 |
| 38 | Carla van Zantvliet (M50) | NED | 53.58 | | +11.590 |
| 39 | Lisa Spruijt (C1) | NED | 53.81 | SB | +11.820 |
| 40 | Julia Groeneveld (D2) | NED | 54.16 | | +12.170 |
| 41 | Lisa Brandt (D2) | NED | 54.31 | SB | +12.320 |
| 42 | Merit de Bruin (D2) | NED | 55.18 | | +13.190 |
| 43 | Nicole Noom (D1) | NED | 55.51 | | +13.520 |
| 44 | Sylvie van den Heuvel (C1) | NED | 55.56 | | +13.570 |
| 45 | Julia-Rosa Jansen (C1) | NED | 56.81 | | +14.820 |
| 46 | Marit.0608 van der Meer (E2) | NED | 58.29 | | +16.300 |
| 47 | Merel Pletting (B1) | NED | 59.67 | SB | +17.680 |
| 48 | Annabel Kolk (C1) | NED | 59.98 | SB | +17.990 |
| 49 | Eveline Krom (D2) | NED | 1:02.65 | SB | +20.660 |
| 50 | Evy van Duijn (E2) | NED | 1:03.28 | SB | +21.290 |
| 51 | Iris Dekker (D1) | NED | 1:08.92 | | +26.930 |
| DQ | Romy Ravestijn (C1) | NED | DQ | | - |