| 1 | Melissa Wijfje (Neo) | NED | 40.00 | SB | - |
| 2 | Nille Martha Norhaug Faleide (Senior) | NOR | 40.68 | | +0.680 |
| 3 | Yu-Ting Huang (Senior) | TPE | 40.91 | NR | +0.910 |
| 4 | Maria Jose Moya (Senior) | CHI | 41.17 | | +1.170 |
| 5 | Linda Koomen (Neo) | NED | 41.68 | | +1.680 |
| 6 | Saskia Alusalu (Neo) | EST | 41.85 | | +1.850 |
| 7 | Maaike Koomen (Neo) | NED | 42.13 | SB | +2.130 |
| 8 | Valentina Moya (Senior) | CHI | 42.73 | | +2.730 |
| 9 | Chantal Müller (B1) | GER | 42.74 | | +2.740 |
| 10 | Kristin Meyer (B1) | GER | 42.95 | | +2.950 |
| 11 | Marit Fjellanger Bøhm (Senior) | NOR | 43.22 | | +3.220 |
| 12 | Viviana Rodriguez (Senior) | CHI | 43.51 | | +3.510 |
| 13 | Anna Molnar (C2) | ROU | 44.99 | SB | +4.990 |
| 14 | Henrike Michalke (B1) | GER | 45.25 | | +5.250 |
| 15 | Edina Szabados (C1) | ROU | 45.94 | SB | +5.940 |
| 16 | Elena Monica Margaritaru (A1) | ROU | 47.44 | | +7.440 |
| 17 | Veronika Lackova (C1) | CZE | 47.71 | | +7.710 |
| 18 | Ashley Völker (D1) | GER | 47.76 | SB | +7.760 |
| 19 | Clea Zimmermann (C2) | GER | 48.43 | | +8.430 |
| 20 | Hana Cechová (D1) | CZE | 49.07 | | +9.070 |
| 21 | Adela Petrusova (D2) | CZE | 49.54 | | +9.540 |
| 22 | Sabine Rothenfußer (D1) | GER | 49.82 | SB | +9.820 |
| 23 | Nora Kästner (D1) | GER | 50.00 | | +10.000 |
| 24 | Daniela Dinn (D2) | ROU | 50.18 | | +10.180 |
| 25 | Nina Mayrhofer (D2) | GER | 50.54 | | +10.540 |
| 26 | Emelia Alexandrescu (D2) | ROU | 50.60 | PB | +10.600 |
| 27 | Anna Arlt (M30) | GER | 50.99 | | +10.990 |
| 28 | Nella Sankova (D2) | CZE | 51.25 | | +11.250 |
| 29 | Isabel Kraus (D1) | GER | 51.46 | | +11.460 |
| 30 | Katerina Macháckova (D1) | CZE | 51.60 | SB | +11.600 |
| Lucia Brero (E2) | GER | 51.60 | SB | +11.600 |
| 32 | Louisa Patz (D2) | GER | 51.93 | | +11.930 |
| 33 | Marie Kadlecová (D1) | CZE | 52.10 | SB | +12.100 |
| 34 | Martina Zemkova (B1) | CZE | 52.85 | | +12.850 |
| 35 | Teodora Párvu (E1) | ROU | 53.02 | | +13.020 |
| 36 | Elena Marschner (D1) | GER | 53.75 | SB | +13.750 |
| 37 | Andreea Moise (E1) | ROU | 54.36 | | +14.360 |
| 38 | Barbora Slámová (F2) | CZE | 55.45 | | +15.450 |
| 39 | Monika Macháckova (E1) | CZE | 56.08 | | +16.080 |
| 40 | Linn Kauer (D2) | GER | 56.17 | PB | +16.170 |
| 41 | Adéla Krejčová (F2) | CZE | 56.52 | | +16.520 |
| 42 | Franziska Siegert (D1) | GER | 57.60 | SB | +17.600 |
| 43 | Ema Schneiderova (E2) | CZE | 57.76 | | +17.760 |
| 44 | Kristýna Němcová (E1) | CZE | 58.91 | SB | +18.910 |
| 45 | Maria Valinescu (E1) | ROU | 1:01.26 | | +21.260 |
| 46 | Magdalena Kern (E2) | GER | 1:01.59 | SB | +21.590 |
| 47 | Melanie Schröcker (E1) | GER | 1:04.96 | SB | +24.960 |
| 48 | Katharina Wieser (E2) | GER | 1:07.34 | PB | +27.340 |
| 49 | Luisa Tillmanns (E1) | GER | 1:13.41 | | +33.410 |
| 50 | Barbora Sábliková (F1) | CZE | 1:19.13 | | +39.130 |
| DNS | Andelka Jaksevic (D1) | GER | DNS | | - |