| 1 | Chae-Eun Park (C2) | KOR | 39.64 | SB | - |
| 2 | Paige Schwartzburg (Senior) | USA | 39.92 | | +0.280 |
| 3 | Rebekah Bradford-Plath (M35) | USA | 40.03 | | +0.390 |
| 4 | Yu-Ting Huang (Senior) | TPE | 40.17 | NR | +0.530 |
| 5 | Brianna Bocox (Neo) | USA | 40.48 | | +0.840 |
| 6 | Franchesca Bell (Neo) | USA | 41.23 | | +1.590 |
| 7 | Maria Victoria Rodriguez (Senior) | ARG | 41.67 | | +2.030 |
| 8 | Jenni Kukkonen (Senior) | FIN | 41.75 | | +2.110 |
| 9 | Esther Munoz (Neo) | USA | 41.97 | | +2.330 |
| 10 | Chrysta Rands (Neo) | USA | 42.08 | | +2.440 |
| 11 | Carlijn Schoutens (Neo) | USA | 42.28 | | +2.640 |
| 12 | Mariah Richardson (Senior) | USA | 42.57 | | +2.930 |
| 13 | Jamie Lindquist-Nielson (A2) | USA | 42.67 | | +3.030 |
| 14 | Saree Han (A2) | USA | 42.79 | | +3.150 |
| 15 | Yun Huang (Senior) | TPE | 42.87 | | +3.230 |
| 16 | Grace Oh (C2) | USA | 43.60 | SB | +3.960 |
| 17 | Hailey Choi (C1) | USA | 43.68 | PB | +4.040 |
| 18 | Lindsey Woodbury (C2) | USA | 43.71 | | +4.070 |
| 19 | Valentina Espinosa (A2) | COL | 44.05 | | +4.410 |
| 20 | Janine Park (D2) | USA | 45.43 | | +5.790 |
| 21 | Abigail Hailey Sorenson (C2) | USA | 46.12 | SB | +6.480 |
| 22 | Laura Gomez Quintero (Senior) | COL | 46.17 | | +6.530 |
| 23 | Eunice Lee (D2) | USA | 46.28 | | +6.640 |
| 24 | Courtney Anderson (A2) | USA | 47.35 | | +7.710 |
| 25 | Grace Lee (D1) | USA | 47.68 | PB | +8.040 |
| 26 | Alexis Havener (C2) | USA | 48.29 | PB | +8.650 |
| 27 | Tessa Lessner (C1) | USA | 50.56 | | +10.920 |
| 28 | Marley Soldan (E2) | USA | 52.78 | SB | +13.140 |
| 29 | Jocylyn Teece (E2) | USA | 1:00.35 | | +20.710 |