| 1 | Isabelle Hersbach (Neo) | NED | 43.46 | | - |
| 2 | Yiran van 'T Oosten (C2) | NED | 45.14 | | +1.680 |
| 3 | Blanche Cardinal Tremblay (B1) | CAN | 45.41 | | +1.950 |
| 4 | Kiki van Schaik (B2) | NED | 46.21 | | +2.750 |
| 5 | Scarlett de Bruijn (D2) | NED | 46.43 | SB | +2.970 |
| 6 | Kem Scheffer (C1) | NED | 47.27 | PB | +3.810 |
| 7 | Nerena van Vuuren (Neo) | NED | 47.42 | | +3.960 |
| 8 | Sanne Knijnenburg (Senior) | NED | 48.04 | SB | +4.580 |
| 9 | Evelien Ras (C2) | NED | 48.54 | SB | +5.080 |
| 10 | Lenthe Jansen (C2) | NED | 48.57 | | +5.110 |
| 11 | Britt Post (D2) | NED | 49.14 | | +5.680 |
| 12 | Ava Alleblas (C1) | NED | 50.08 | PB | +6.620 |
| 13 | Bente Barendse (C1) | NED | 50.23 | | +6.770 |
| 14 | Kimberley Hanemaayer (Senior) | NED | 50.33 | SB | +6.870 |
| 15 | Lies Prinssen (D2) | NED | 50.51 | PB | +7.050 |
| 16 | Hilde Dijkers (B2) | NED | 50.59 | PB | +7.130 |
| 17 | Janneke van den Bos (M35) | NED | 51.02 | | +7.560 |
| 18 | Valerie Gerritse (D1) | NED | 52.35 | | +8.890 |
| 19 | Esmee van der Meer (C2) | NED | 52.95 | | +9.490 |
| 20 | Evy Baars (C2) | NED | 53.16 | SB | +9.700 |
| 21 | Feline Solleveld (D2) | NED | 53.40 | SB | +9.940 |
| 22 | Clover van Zeijl (D2) | NED | 53.47 | SB | +10.010 |
| 23 | Evi Lagerwerf (D2) | NED | 53.64 | SB | +10.180 |
| 24 | Senne Te Nijenhuis (C1) | NED | 53.93 | SB | +10.470 |
| 25 | Mirthe Wijnakker (D2) | NED | 54.13 | SB | +10.670 |
| 26 | Anne-Lotte Kohler (D2) | NED | 54.20 | | +10.740 |
| 27 | Lindsey Eijgermans (D2) | NED | 54.27 | SB | +10.810 |
| 28 | Francis de Bruijn (E2) | NED | 55.15 | | +11.690 |
| 29 | Oona Alleblas (D1) | NED | 55.16 | | +11.700 |
| 30 | Maaike van Zijll (Senior) | NED | 55.23 | SB | +11.770 |
| 31 | Anne Van Kester (n/a) | NED | 55.52 | PB | +12.060 |
| 32 | Melissa Buijnink (Senior) | NED | 55.71 | SB | +12.250 |
| 33 | Jolijn Solleveld (E2) | NED | 56.02 | | +12.560 |
| 34 | Maaike Helleman (D1) | NED | 56.33 | SB | +12.870 |
| 35 | Julia Van Der Voort (n/a) | NED | 56.96 | PB | +13.500 |
| 36 | Nanette de Boon (E2) | NED | 57.13 | | +13.670 |
| 37 | Simone De Jong (M30) | NED | 57.47 | SB | +14.010 |
| 38 | Marian Bosch-Rekveldt (M45) | NED | 58.41 | SB | +14.950 |
| 39 | Elske van Raaphorst (Senior) | NED | 59.18 | SB | +15.720 |
| 40 | Medde Barendse (E2) | NED | 1:00.48 | SB | +17.020 |
| 41 | Madelief Knol (D1) | NED | 1:01.95 | PB | +18.490 |
| 42 | Roos Weber (M55) | NED | 1:02.39 | SB | +18.930 |
| 43 | Ellen van Toor (M60) | NED | 1:06.80 | SB | +23.340 |
| 44 | Anne-Fleur Knol (D1) | NED | 1:12.94 | | +29.480 |
| 45 | Lizelot Vergouwe (n/a) | NED | 1:22.05 | PB | +38.590 |