| 1 | Mirjam Tuls (Neo) | NED | 43.91 | | - |
| 2 | Sophie Scheer (Neo) | NED | 44.88 | | +0.970 |
| 3 | Anne Heijkoop (Neo) | NED | 44.96 | SB | +1.050 |
| 4 | Manouk `t Mannetje (Senior) | NED | 45.06 | | +1.150 |
| 5 | Lijnie Boele (M40) | NED | 45.58 | | +1.670 |
| 6 | Sophie Moerman (Senior) | NED | 45.99 | | +2.080 |
| 7 | Eline Lagendijk (Neo) | NED | 46.03 | | +2.120 |
| 8 | Hilleke Booij (Neo) | NED | 46.70 | | +2.790 |
| 9 | Erna Hilgersom (Senior) | NED | 46.76 | | +2.850 |
| 10 | Esther van Galen (Senior) | NED | 46.78 | | +2.870 |
| 11 | Jolanda Voskamp-Vollebregt (M40) | NED | 48.09 | | +4.180 |
| 12 | Mariëlle Koek (Senior) | NED | 48.73 | | +4.820 |
| 13 | Marianne de Neeling (M50) | NED | 49.04 | | +5.130 |
| 14 | Roos-Marijn Kinkel (Senior) | NED | 49.29 | SB | +5.380 |
| 15 | Jolanda de Haan (M40) | NED | 49.93 | | +6.020 |
| 16 | Marloes Gravesteijn (Senior) | NED | 50.07 | | +6.160 |
| 17 | Marleen Smeulers (M45) | NED | 51.26 | | +7.350 |
| 18 | Corina Slingerland-van Zwienen (M45) | NED | 51.57 | | +7.660 |
| 19 | Netty de Bruijn (M40) | NED | 51.94 | | +8.030 |
| 20 | Ellie van Hagen-Boelsma (M55) | NED | 53.30 | | +9.390 |
| 21 | Letty Ruhaak (M55) | NED | 54.65 | SB | +10.740 |
| 22 | Els de Zwart (M60) | NED | 56.60 | SB | +12.690 |
| 23 | Evelien van der Schuur (Senior) | NED | 57.17 | | +13.260 |
| 24 | José van Viersen (M45) | NED | 1:05.70 | | +21.790 |