| 1 | Joanne Spliethof (M30) | NED | 45.79 | | - |
| 2 | Femke Assink (Neo) | NED | 45.96 | | +0.170 |
| 3 | Antoinet van Essen-Prins (M30) | NED | 47.02 | | +1.230 |
| 4 | Lenneke Groot Koerkamp (A1) | NED | 47.23 | SB | +1.440 |
| 5 | Janneke Bos (B2) | NED | 48.85 | | +3.060 |
| 6 | Annabel Weteringe (B1) | NED | 48.91 | PB | +3.120 |
| 7 | Milou Klatt-ten Cate (M35) | NED | 49.32 | SB | +3.530 |
| 8 | Lisa Otten (C2) | NED | 49.70 | | +3.910 |
| 9 | Inge Pelgröm (Neo) | NED | 49.81 | SB | +4.020 |
| 10 | Isa van der Weerd (D2) | NED | 50.69 | SB | +4.900 |
| 11 | Bente van Dasselaar (C1) | NED | 50.78 | | +4.990 |
| 12 | Marit Noordman (B1) | NED | 51.39 | SB | +5.600 |
| 13 | Sybrich Hillie Buisman (D2) | NED | 51.43 | | +5.640 |
| 14 | Lena Wisselink (D2) | NED | 52.56 | | +6.770 |
| 15 | Jill Krikken (E2) | NED | 52.68 | | +6.890 |
| 16 | Dione Groot Jebbink (D2) | NED | 54.51 | | +8.720 |
| 17 | Saar Burgwal (D2) | NED | 54.77 | SB | +8.980 |
| 18 | Neyómy Barry (D2) | NED | 54.99 | | +9.200 |
| 19 | Jinke van der Velde (D2) | NED | 55.25 | PB | +9.460 |
| 20 | Silke van den Bos (C1) | NED | 55.56 | | +9.770 |
| 21 | Eva Mezzo (D1) | NED | 55.80 | | +10.010 |
| 22 | Iris Noordman (D2) | NED | 55.95 | | +10.160 |
| 23 | Tessa Bosman (C2) | NED | 1:04.45 | | +18.660 |
| DQ | Elin Mak (E1) | NED | DQ | | - |
| DQ | Mireille van der Weerd (C2) | NED | DQ | | - |