| 1 | Judith Dannhauer (Senior) | GER | 39.02 | | - |
| 2 | Elina Risku (Senior) | FIN | 39.20 | SB | +0.180 |
| 3 | Yvonne Daldossi (Senior) | ITA | 39.95 | | +0.930 |
| 4 | Kaja Ziomek (A2) | POL | 40.04 | | +1.020 |
| 5 | Martina Sáblíková (Senior) | CZE | 40.17 | | +1.150 |
| 6 | Nikola Zdráhalova (Neo) | CZE | 40.20 | | +1.180 |
| 7 | Maud Lugters (B1) | NED | 40.63 | | +1.610 |
| 8 | Claudia Pechstein (Senior) | GER | 40.90 | | +1.880 |
| Aleksandra Kapruziak (Neo) | POL | 40.90 | SB | +1.880 |
| 10 | Anne Ruyssenaars (A2) | NED | 40.91 | PB | +1.890 |
| 11 | Anouk Karel (Neo) | NED | 41.80 | | +2.780 |
| 12 | Mariin Sarap (Neo) | EST | 41.95 | PB | +2.930 |
| 13 | Eliska Drimalova (Neo) | CZE | 41.99 | SB | +2.970 |
| 14 | Victoria Stirnemann (C2) | GER | 42.05 | | +3.030 |
| 15 | Elsemieke van Maaren (Neo) | NED | 42.14 | SB | +3.120 |
| 16 | Amber Bartelds (B2) | NED | 42.27 | | +3.250 |
| 17 | Miina Peltonen (Neo) | FIN | 42.40 | | +3.380 |
| 18 | Natálie Kerschbaummayr (A2) | CZE | 42.54 | SB | +3.520 |
| 19 | Janetta Tolsma (Neo) | NED | 42.65 | SB | +3.630 |
| Lonneke Uneken (B2) | NED | 42.65 | | +3.630 |
| 21 | Marit van Noort (A1) | NED | 42.86 | PB | +3.840 |
| 22 | Iga Wojtasik (B2) | POL | 43.09 | | +4.070 |
| 23 | Nathalie Dijs (A1) | NED | 43.24 | | +4.220 |
| 24 | Ju-Lin de Visser (B1) | NED | 43.67 | | +4.650 |
| 25 | Ruth Balvert (B1) | NED | 43.75 | | +4.730 |
| 26 | Laura Kivioja (C1) | FIN | 43.78 | SB | +4.760 |
| 27 | Carmen van der Peet (B2) | NED | 44.55 | | +5.530 |
| 28 | Katriina Keski-Opas (A1) | FIN | 44.66 | | +5.640 |
| Anni Käsnänen (A1) | FIN | 44.66 | | +5.640 |
| 30 | Jolanda Meier (Neo) | NED | 44.71 | | +5.690 |
| 31 | Quinty Ton (A1) | NED | 45.05 | SB | +6.030 |
| 32 | Fleur Biesbroek (C2) | NED | 45.27 | | +6.250 |
| 33 | Tilda-Sofiia Oras (A1) | FIN | 46.16 | SB | +7.140 |
| 34 | Emmy Pordon (A1) | NED | 47.85 | | +8.830 |
| 35 | Linda Lankhorst (D2) | NED | 47.90 | | +8.880 |
| 36 | Alessandra Franco (A2) | ITA | 48.45 | SB | +9.430 |
| DNS | Katja Franzen (Senior) | GER | DNS | | - |