| 1 | Katja Franzen (Senior) | GER | 39.24 | SB | - |
| 2 | Anna Ostlender (A1) | GER | 40.49 | | +1.250 |
| 3 | Josephine Heimerl (A2) | GER | 40.52 | | +1.280 |
| 4 | Meghan Wystrichowski (A1) | GER | 40.94 | | +1.700 |
| 5 | Jenny Peißker (Senior) | GER | 41.02 | | +1.780 |
| 6 | Sophie Warmuth (B2) | GER | 41.09 | | +1.850 |
| 7 | Laura Kivioja (B2) | FIN | 41.26 | NRJ | +2.020 |
| 8 | Luisa Gonzalez Salazar (B1) | ESP | 41.45 | NRJ, NR | +2.210 |
| 9 | Laethisia Schimek (Senior) | GER | 41.83 | SB | +2.590 |
| 10 | Maira Jasch (C2) | GER | 41.91 | | +2.670 |
| 11 | Ilka Fuzesy (B1) | ROU | 41.93 | | +2.690 |
| 12 | Hanna Biró (B1) | HUN | 42.00 | | +2.760 |
| 13 | Christl Anders (Neo) | GER | 42.14 | SB | +2.900 |
| 14 | Kristiine Kalev (Neo) | EST | 42.61 | | +3.370 |
| 15 | Teresa Moreno (Neo) | ESP | 42.91 | SB | +3.670 |
| 16 | Tilda-Sofiia Oras (Neo) | FIN | 43.10 | SB | +3.860 |
| 17 | Leonie Mayrhofer (B1) | GER | 43.32 | | +4.080 |
| 18 | Nerea Langa (A2) | ESP | 43.52 | | +4.280 |
| 19 | Emily Hohendorf (C2) | GER | 43.55 | | +4.310 |
| 20 | Steffi Rothenfußer (A2) | GER | 43.75 | | +4.510 |
| 21 | Alexa Kebschull (B2) | GER | 43.87 | | +4.630 |
| 22 | Daria Hurzhii (B1) | UKR | 43.92 | NRJ | +4.680 |
| 23 | Nina Mayrhofer (B1) | GER | 43.95 | | +4.710 |
| 24 | Marlen Ehseluns (B2) | GER | 44.13 | | +4.890 |
| 25 | Julia Espin Real (C1) | ESP | 44.16 | SB | +4.920 |
| 26 | Bibiane Werner (C2) | GER | 44.48 | | +5.240 |
| 27 | Janice Heimberger (B2) | GER | 44.65 | | +5.410 |
| 28 | Carla Alvarez Fernandez (B1) | ESP | 44.82 | | +5.580 |
| 29 | Rebeka Vancsó (D2) | HUN | 44.89 | NRJ | +5.650 |
| 30 | Lilla Malinovszky (A1) | HUN | 45.02 | | +5.780 |
| 31 | Josephine Mönnich (A1) | GER | 45.13 | | +5.890 |
| 32 | Nikolett Rigó (A1) | HUN | 45.21 | SB | +5.970 |
| Jamie Palilaja (Neo) | IDN | 45.21 | | +5.970 |
| 34 | Ashley Völker (C2) | GER | 45.58 | | +6.340 |
| 35 | Elizabeth Lazaridou (C2) | GER | 45.99 | | +6.750 |
| 36 | Hanna Talabèr (B2) | HUN | 46.00 | | +6.760 |
| 37 | Laura Altenhof (B1) | GER | 46.35 | | +7.110 |
| 38 | Hanna Müller (B1) | AUT | 46.60 | | +7.360 |
| 39 | Julia Geutner (C1) | GER | 46.86 | | +7.620 |
| 40 | Petra Zsankó (A2) | HUN | 46.90 | PB | +7.660 |
| 41 | Hannah Llop Izquierdo (C2) | ESP | 46.94 | | +7.700 |
| 42 | Gina Knackstedt (C2) | GER | 47.02 | | +7.780 |
| 43 | Barbora Slámová (D1) | CZE | 47.24 | | +8.000 |
| 44 | Viktorija Gritsenko (A1) | UKR | 47.34 | | +8.100 |
| 45 | Adéla Krejčová (D1) | CZE | 47.50 | | +8.260 |
| 46 | Sabine Rothenfußer (C2) | GER | 47.65 | | +8.410 |
| 47 | Alexandra Snowdon (D2) | GBR | 47.69 | NRJ | +8.450 |
| 48 | Hanna Hnatiuk (C1) | UKR | 47.96 | SB | +8.720 |
| 49 | Monika Macháckova (D2) | CZE | 48.06 | | +8.820 |
| 50 | Fanni Palakovics (D2) | HUN | 48.14 | SB | +8.900 |
| 51 | Jessica Uhlemann (Senior) | GER | 48.17 | | +8.930 |
| 52 | Ema Schneiderova (C1) | CZE | 48.33 | | +9.090 |
| 53 | Sheila Gómez Fernandez (B1) | ESP | 48.51 | SB | +9.270 |
| 54 | Kira Amschl (E2) | HUN | 48.72 | PB | +9.480 |
| 55 | Charline Coates-Kwadrin (D2) | GBR | 49.27 | | +10.030 |
| 56 | Szofi Balaton (D2) | HUN | 49.38 | | +10.140 |
| 57 | Kerli Prohorenko (C1) | EST | 49.86 | | +10.620 |
| 58 | Kristýna Němcová (D2) | CZE | 49.93 | | +10.690 |
| 59 | Ina Völker (M40) | GER | 50.00 | | +10.760 |
| 60 | Philippa Draper (B1) | GBR | 50.18 | PB | +10.940 |
| 61 | Viktoria Eistre (B1) | EST | 51.95 | | +12.710 |
| 62 | Emilia Hopf (E1) | GER | 52.18 | | +12.940 |
| 63 | Zsuzsanna Mórotz (D1) | HUN | 52.59 | SB | +13.350 |
| 64 | Gabriela Ctvrtkova (E2) | CZE | 53.49 | | +14.250 |
| 65 | Johanna Scheucher (D1) | GER | 55.84 | | +16.600 |
| 66 | Lucie Nemcova (E1) | CZE | 59.67 | | +20.430 |
| 67 | Vanesa Hyzova (F2) | CZE | 1:01.40 | SB | +22.160 |
| 68 | Anezka Skupinová (D2) | CZE | 1:01.90 | | +22.660 |
| 69 | Gabriela Pataki (E1) | CZE | 1:02.24 | | +23.000 |
| 70 | Anna Duronová (E1) | CZE | 1:05.39 | | +26.150 |
| DQ | Getter Rebane (A1) | EST | DQ | | - |
| DNS | Pia-Leonie Kirsakal (A2) | GER | DNS | | - |
| WDR | Isabel Kraus (C2) | GER | WDR | | - |